ELISA Untuk Keamanan Pangan

Published by Administrator at February 21, 2019

Keracunan Pangan

Penyakit yang disebabkan makanan (atau sering disebut keracunanan makanan) adalah semua penyakit yang timbul karena makanan yang terkontaminasi. Penyebab utama keracunan makanan adalah bakteri dan virus berbahaya, parasit, pengawet makanan dan kontaminan lainnya.

Baca juga artikel lainnya mengenai ELISA Kit Untuk Penelitian Kanker di sini

Di Indonesia sendiri, selama tahun 2017 Badan POM telah mencatat 57 berita keracunan pangan yang diperoleh dari media massa dan PHEOC (Pos Kedaruratan Kesehatan Masyarakat atau Public Health Emergency Operating Center) . Sementara di tahun yang sama, sebanyak 53 kejadian luar biasa (KLB) keracunan pangan dilaporkan oleh 34 BB/BPOM di seluruh Indonesia. Laporan tersebut diperoleh dari Dinas Kesehatan Propinsi maupun Kabupaten/Kota di 34 Propinsi.

Gambar 1. Profil Kejadian dan Kasus KLB Keracunan Pangan Tahun 2017

Dilaporkan jumlah orang yang terpapar sebanyak 5293 orang, sedangkan kasus KLB keracunan pangan (case) yang dilaporkan sebanyak 2041 orang sakit dan 3 orang meninggal dunia. Berdasarkan data tersebut diketahui nilai Attack Rate (AR) sebesar 38,56% dan Case Fatality Rate (CFR) sebesar 0,15%. Attack Rate merupakan jumlah kasus pada periode KLB dibagi dengan jumlah yang mengkonsumsi dikalikan 100. Case Fatality Rate merupakan jumlah korban meninggal dibagi jumlah kasus selama periode KLB dikali dengan 100. Ditinjau dari segi etiologi, penyebab KLB Keracunan Pangan dapat dilihat pada Gambar berikut.

Gambar 2. Penyebab KLB Keracunan Pangan Tahun 2017

Keamanan Pangan

Makanan dapat memindahkan penyakit karena makanan yang disajikan tersebut digunakan oleh bakteri sebagai media tumbuh dan mengeluarkan racun yang berbahaya bagi kesehatan. Keamanan pangan dapat diartikan sebagai makanan yang non-toksik, tidak berbahaya, dengan tetap mengandung nutrisi yang dibutuhkan, dan tidak menyebabkan bahaya pada kesehatan manusia, baik level akut, sub akut atau kronis. Sekarang ini perhatian terhadap keamanan pangan terus berkembang di seluruh dunia terutama di negara berkembang.

Solusi untuk Keamanan Pangan

Pengujian pangan memiliki peran penting dalam kontrol kualitas selama proses produksi. Tes kit pangan menjadikan pengujian pangan lebih efektif dan efisien.

ELISA merupakan metode deteksi yang dapat mendeteksi secara kualitatif atau quantitative bakteri, parasit, bahan kimia, residu obat atau kontaminan pangan lainnya. Elabscience® menyediakan bermacam ELISA kit untuk mendeteksi kontaminan pangan secara quantitative. Kit ELISA Elabscience® ini dapat digunakan untuk pengujian makanan atau pun juga pakan ternak dengan dasar teknologi immunologi.

Sulfonamides

–	–	–	–	–
Detection target	Catalogue Number	Product name	Sensitivity	Sample type
Sulfamethazine	E-FS-E043	SM2 (Sulfamethazine) ELISA Kit	0.5 ppb	Tissue, Serum, Honey, Milk, Egg, Urine
Sulfamethoxazole	E-FS-E021	SMZ (Sulfamethoxazole) ELISA Kit	0.1 ppb	Tissue, Serum, Urine, Egg, Honey, Milk, Fodder
Sulfametoxydiazine	E-FS-E052	SMD (Sulfametoxydiazine) ELISA Kit	0.05 ppb	Tissue, Serum, Urine, Honey, Milk
Sulfamonomethoxine	E-FS-E051	SMM (Sulfamonomethoxine) ELISA Kit	0.05 ppb	Tissue, Serum, Urine, Honey, Milk
Sulfaquinoxaline	E-FS-E050	SQX (Sulfaquinoxaline) ELISA Kit	0.5 ppb	Tissue, Serum, Urine, Honey, Milk, Fodder
Sulfonamides	E-FS-E049	SAs (Sulfonamides) ELISA Kit	0.5 ppb	Tissue, Serum, Urine, Honey, Milk

	E-FS-E040	SAs (Sulfonamides) ELISA Kit	0.1 ppb	Tissue, Serum, Urine, Egg, Honey, Milk, Fodder
	E-FS-E039	SAs (Sulfonamides) ELISA Kit	0.5 ppb	Tissue, Serum, Urine, Honey, Milk
	E-FS-E038	SAs (Sulfonamides) ELISA Kit	0.5 ppb	Tissue, Serum, Urine, Egg, Honey, Milk
	E-FS-E037	SAs (Sulfonamides) ELISA Kit	0.5 ppb	Tissue, Serum, Urine, Egg, Honey, Milk, Water sample
	E-FS-C033	SAs (Sulfonamides) Rapid Test Kit	2ppb	Tissue
			(SDZ)
	E-FS-C028	SAs (Sulfonamides) Rapid Test Kit	20 ppb(SM2)	Tissue, Honey, Milk
Trimethoprim	E-FS-E023	TMP (Trimethoprim) ELISA Kit	0.015 ppb	Fodder, Tissue, Serum, Urine, Plasma



	E-FS-E020	TMP (Trimethoprim) ELISA Kit	0.03 ppb	Fodder, Tissue, Serum, Urine, Plasma

Nitrofuran

–	–	–	–	–
Detection target	Catalogue Number	Product name	Sensitivity	Sample type
Sulfamethazine	E-FS-E043	SM2 (Sulfamethazine) ELISA Kit	0.5 ppb	Tissue, Serum, Honey, Milk, Egg, Urine
Sulfamethoxazole	E-FS-E021	SMZ (Sulfamethoxazole) ELISA Kit	0.1 ppb	Tissue, Serum, Urine, Egg, Honey, Milk, Fodder
Sulfametoxydiazine	E-FS-E052	SMD (Sulfametoxydiazine) ELISA Kit	0.05 ppb	Tissue, Serum, Urine, Honey, Milk
Sulfamonomethoxine	E-FS-E051	SMM (Sulfamonomethoxine) ELISA Kit	0.05 ppb	Tissue, Serum, Urine, Honey, Milk
Sulfaquinoxaline	E-FS-E050	SQX (Sulfaquinoxaline) ELISA Kit	0.5 ppb	Tissue, Serum, Urine, Honey, Milk, Fodder
Sulfonamides	E-FS-E049	SAs (Sulfonamides) ELISA Kit	0.5 ppb	Tissue, Serum, Urine, Honey, Milk
	E-FS-E040	SAs (Sulfonamides) ELISA Kit	0.1 ppb	Tissue, Serum, Urine, Egg, Honey, Milk, Fodder
	E-FS-E039	SAs (Sulfonamides) ELISA Kit	0.5 ppb	Tissue, Serum, Urine, Honey, Milk
	E-FS-E038	SAs (Sulfonamides) ELISA Kit	0.5 ppb	Tissue, Serum, Urine, Egg, Honey, Milk
	E-FS-E037	SAs (Sulfonamides) ELISA Kit	0.5 ppb	Tissue, Serum, Urine, Egg, Honey, Milk, Water sample
	E-FS-C033	SAs (Sulfonamides) Rapid Test Kit	2 ppb(SDZ)	Tissue
	E-FS-C028	SAs (Sulfonamides) Rapid Test Kit	20 ppb(SM2)	Tissue, Honey, Milk
Trimethoprim	E-FS-E023	TMP (Trimethoprim) ELISA Kit	0.015 ppb	Fodder, Tissue, Serum, Urine, Plasma
	E-FS-E020	TMP (Trimethoprim) ELISA Kit	0.03 ppb	Fodder, Tissue, Serum, Urine, Plasma

Quinolones

–	–	–	–	–
Detection target	Catalogue Number	Product Name	Sensitivity	Sample type
Quinolones	E-FS-C027	QNs (Quinolones ) Rapid Test Kit	0.5 ppb(ENR)	Tissue, Honey
	E-FS-C034	QNs (Quinolones ) Rapid Test Kit	2 ppb(ENR)	Tissue
	E-FS-C036	QNs (Quinolones ) Rapid Test Kit	5 ppb(ENR)	Tissue
	E-FS-E034	QNs (Quinolones) ELISA Kit	0.1 ppb	Honey, Tissue, Milk (liquid,powder), Egg, Urine
	E-FS-E055	QNs (Quinolones) ELISA Kit	0.2 ppb	Tissue, Honey, Milk (liquid,powder), Egg, Urine
Fluoroquinolones	E-FS-E053	FQNs (Fluoroquinolones) ELISA Kit	0.1 ppb	Tissue, Honey, Milk (liquid,powder), Egg, Urine
	E-FS-E054	FQNs (Fluoroquinolones) ELISA Kit	0.2 ppb	Tissue, Honey, Milk (liquid,powder), Egg, Urine
Enrofloxacin	E-FS-E032	ENR (Enrofloxacin) ELISA Kit	0.1 ppb	Tissue, Honey, Milk (liquid,powder), Egg
	E-FS-E056	ENR (Enrofloxacin) ELISA Kit	0.2 ppb	Tissue, Honey, Milk (liquid,powder), Egg, Urine
Ciprofloxacin	E-FS-C035	CPFX (Ciprofloxacin) Rapid Test Kit	1 ppb	Tissue, Honey
	E-FS-E033	CPFX (Ciprofloxacin) ELISA Kit	0.1 ppb	Tissue, Honey, Milk (liquid,powder), Egg

Lean meat powder

–	–	–	–	–
Detection target	Catalogue Number	Product name	Sensitivity	Sample type
Salbutamol	E-FS-C010	SAL (Salbutamol) Rapid Test Kit	5 ppb	Urine, Fodder
	E-FS-C015	SAL (Salbutamol) Rapid Test Kit	10 ppb	Tissue
	E-FS-E017	SAL (Salbutamol) ELISA Kit	0.1 ppb	Urine, Tissue, Fodder
Ractopamine	E-FS-C008	RAC (Ractopamine) Rapid Test Kit	3 ppb	Urine, Fodder
	E-FS-C014	RAC (Ractopamine) Rapid Test Kit	8 ppb	Tissue
	E-FS-E012	RAC (Ractopamine) ELISA Kit	0.1 ppb	Urine, Tissue, Liver, Fodder
Clenbuterol-Ractopamine-Salbutamol	E-FS-C005	CLE-RAC-SAL (Clenbuterol-Ractopamine-Salbutamol) Rapid Test Kit	3 ppb/3 ppb/5 ppb	Urine, Fodder
	E-FS-C016	CLE-RAC-SAL (Clenbuterol-Ractopamine-Salbutamol) Rapid Test Kit	3 ppb/8 ppb/10 ppb	Tissue
	E-FS-C017	CLE-RAC-SAL (Clenbuterol-Ractopamine-Salbutamol) Rapid Test Kit	2 ppb/3 ppb/3 ppb	Urine, Fodder
Clenbuterol	E-FS-C006	CLE (Clenbuterol) Rapid Test Kit	3 ppb	Urine, Fodder
	E-FS-C013	CLE (Clenbuterol) Rapid Test Kit	3 ppb	Tissue
	E-FS-E014	CLE (Clenbuterol) ELISA Kit	0.1 ppb	Urine, Tissue, Fodder
	E-FS-E025	CLE (Clenbuterol) ELISA Kit	0.05 ppb	Urine, Tissue, Fodder

Others

–	–	–	–	–
Detection target	Catalogue Number	Product name	Sensitivity	Sample type
Tetracycline	E-FS-C030	TCs (Tetracycline) Rapid Test Kit	10 ppb	Tissue, Honey
	E-FS-C031	TCs (Tetracycline) Rapid Test Kit	10 ppb	Milk
	E-FS-E041	TCs (Tetracycline) ELISA Kit	0.3 ppb	Tissue, Liver, Egg, Honey, Urine
	E-FS-E046	TCs (Tetracycline) ELISA Kit	0.05 ppb	Tissue, Liver, Egg, Honey, Urine
Sudan Red	E-FS-E016	SUD (Sudan Red) ELISA Kit	0.3 ppb	Tomato juice, Ketchup, Sambal, Paprika, Fodder, Egg
Streptomycin	E-FS-E031	SM (Streptomycin) ELISA Kit	0.1 ppb	Tissue, Honey, Milk
Sarafloxacin	E-FS-E022	SAR (Sarafloxacin) ELISA Kit	0.1 ppb	Tissue, Honey, Milk(liquid,powder)
Phenylethanolamine A	E-FS-C011	PHEA (Phenylethanolamine A) Rapid Test Kit	3 ppb	Urine
	E-FS-E015	PHEA (Phenylethanolamine A) ELISA Kit	0.1 ppb	Urine, Tissue, Fodder
Olaquindox	E-FS-E007	OQX (Olaquindox) ELISA Kit	0.5 ppb	Tissue, Fodder
Olaquindox	E-FS-E008	MQCA (Methyl-3-quinoxaline-2-car-boxylic acid) ELISA Kit	0.3 ppb	Muscle, Pork liver
Nitroimidazole	E-FS-E035	NMZ (Nitroimidazole) ELISA Kit	0.5 ppb	Tissue, Honey
	E-FS-E011	MNZ (Metronidazole) ELISA Kit	0.5 ppb	Tissue, Honey
Melamine	E-FS-C009	MEL (Melamine) Rapid Test Kit	500 ppb	Raw milk, Milk
	E-FS-C012	MEL (Melamine) Rapid Test Kit	100ppb	Raw milk, Milk
	E-FS-E010	MEL (Melamine) ELISA Kit	2 ppb	Milk(liquid,powder), Fodder, Tissue, Egg, Serum
	E-FS-E019	MEL (Melamine) ELISA Kit	1 ppb	Milk(liquid,powder), Fodder, Tissue, Egg, Serum
Malachite Green	E-FS-C007	MG (Malachite Green) Rapid Test Kit	1 ppb/2 ppb	Tissue, Water sample
	E-FS-E013	MG (Malachite Green) ELISA Kit	0.025 ppb	Fish,Shrimp
Diethylstilbestrol	E-FS-E001	DES (Diethylstilbestrol) ELISA Kit	0.05 ppb	Tissue, Pork,Liver, Chichen,Liver
Diazepam	E-FS-E027	DZP (Diazepam) ELISA Kit	0.3 ppb	Tissue, Urine, Fodder
Dexamethasone	E-FS-E009	DEX (Dexamethasone) ELISA Kit	0.1 ppb	Muscle tissue, Milk, Fodder
Cimaterol	E-FS-E026	CIM (Cimaterol) ELISA Kit	0.3 ppb	Urine, Tissue, Fodder
Chlorotetracycline	E-FS-E024	CTC (Chlorotetracycline) ELISA Kit	0.1 ppb	Tissue, Liver, Egg, Honey, Urine
Chloramphenicol	E-FS-C026	CAP (Chloramphenicol) Rapid Test Kit	0.3 ppb	Milk, Tissue, Honey
	E-FS-E030	CAP (Chloramphenicol) ELISA Kit	0.05 ppb	Tissue, Liver, Honey, Milk(liquid,powder), Egg, Water sample, Urine, Serum, Casing, Fodder
	E-FS-E044	CAP (Chloramphenicol) ELISA Kit	0.025 ppb	Tissue, Liver, Honey, Milk(liquid,powder), Egg, Water sample, Urine, Serum, Casing, Fodder
Amantadine	E-FS-E006	Am (Amantadine) ELISA Kit	0.5 ppb	Tissue

Sumber Literatur

Brosur Elabscience
Laporan tahunan BPOM 2017 (web version)

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